cd131 pe Search Results


cd131 csf2rb il3rb il5rb antibody pe mouse mab  (Sino Biological)
N/A
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant Human CD131 CSF2RB IL3RB IL5RB rh CD131 CSF2RB IL3RB
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anti-cd131 antibody-pe  (TargetMol)
N/A
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pe rat anti-mouse cd131  (Becton Dickinson)
90
Becton Dickinson pe rat anti-mouse cd131
(A) TonB cells cultured in the presence of IL-3 or doxycycline were spun down on glass slides, fixed by ice-cold methanol and stained sequentially with anti-STAT5B, anti-rabbit-FITC and DAPI. Intracellular localization was analyzed using confocal microscopy. Nuclear localization was confirmed by a DNA-specific DAPI counterstain. The lower panel shows antibody (with and without enhancement) and DAPI control. (B) TonB cells expressing STAT5A-eGFP were cultured in the presence of IL-3, BCR-ABL or BCR-ABL plus imatinib mesylate (BCR-ABL+IM) and live-cell analysis was performed by confocal microscopy (upper panel). The middle panel shows STAT5A-eGFP in BCR-ABL-expressing TonB cells after removal of imatinib mesylate (−IM) for the indicated periods of time. The lower panel shows empty vector control (eGFP). (C) TonB-STAT5A-eGFP cells treated as in , middle panel, were fixed 45 minutes after imatinib-removal, stained with <t>anti-CD131-PE</t> antibody (IL-3/IL-5/GM-CSF-receptor common β-chain) and DAPI before analysis by confocal microscopy.
Pe Rat Anti Mouse Cd131, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd131+pe/anti+cd131+pe/pmc04023949-64-19-25
Average 90 stars, based on 1 article reviews
pe rat anti-mouse cd131 - by Bioz Stars, 2026-09
90/100 stars
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pe mouse anti human cd131  (BD Biosciences)
N/A
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pe anti-human cd131  (BioLegend)
N/A
PE anti-human CD131 [1C1]; Isotype: Mouse IgG1, κ; Reactivity: Human; Apps: FC; Size: 100 tests
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cd131 / csf2rb / il3rb / il5rb antibody (pe), mouse mab  (Sino Biological)
N/A
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD131 / CSF2RB / IL3RB / IL5RB (rh
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pe rat anti mouse cd131  (BD Biosciences)
N/A
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Image Search Results


(A) TonB cells cultured in the presence of IL-3 or doxycycline were spun down on glass slides, fixed by ice-cold methanol and stained sequentially with anti-STAT5B, anti-rabbit-FITC and DAPI. Intracellular localization was analyzed using confocal microscopy. Nuclear localization was confirmed by a DNA-specific DAPI counterstain. The lower panel shows antibody (with and without enhancement) and DAPI control. (B) TonB cells expressing STAT5A-eGFP were cultured in the presence of IL-3, BCR-ABL or BCR-ABL plus imatinib mesylate (BCR-ABL+IM) and live-cell analysis was performed by confocal microscopy (upper panel). The middle panel shows STAT5A-eGFP in BCR-ABL-expressing TonB cells after removal of imatinib mesylate (−IM) for the indicated periods of time. The lower panel shows empty vector control (eGFP). (C) TonB-STAT5A-eGFP cells treated as in , middle panel, were fixed 45 minutes after imatinib-removal, stained with anti-CD131-PE antibody (IL-3/IL-5/GM-CSF-receptor common β-chain) and DAPI before analysis by confocal microscopy.

Journal: PLoS ONE

Article Title: BCR-ABL Affects STAT5A and STAT5B Differentially

doi: 10.1371/journal.pone.0097243

Figure Lengend Snippet: (A) TonB cells cultured in the presence of IL-3 or doxycycline were spun down on glass slides, fixed by ice-cold methanol and stained sequentially with anti-STAT5B, anti-rabbit-FITC and DAPI. Intracellular localization was analyzed using confocal microscopy. Nuclear localization was confirmed by a DNA-specific DAPI counterstain. The lower panel shows antibody (with and without enhancement) and DAPI control. (B) TonB cells expressing STAT5A-eGFP were cultured in the presence of IL-3, BCR-ABL or BCR-ABL plus imatinib mesylate (BCR-ABL+IM) and live-cell analysis was performed by confocal microscopy (upper panel). The middle panel shows STAT5A-eGFP in BCR-ABL-expressing TonB cells after removal of imatinib mesylate (−IM) for the indicated periods of time. The lower panel shows empty vector control (eGFP). (C) TonB-STAT5A-eGFP cells treated as in , middle panel, were fixed 45 minutes after imatinib-removal, stained with anti-CD131-PE antibody (IL-3/IL-5/GM-CSF-receptor common β-chain) and DAPI before analysis by confocal microscopy.

Article Snippet: Cells were blocked in PBS supplemented with 10% [v/v] FCS (Biochrome, Berlin, Germany) for 1 hour and stained with PE Rat anti-mouse CD131 (559920) from BD Pharmingen and DAPI (0.5 µg/mL) for 2 hours.

Techniques: Cell Culture, Staining, Confocal Microscopy, Expressing, Plasmid Preparation